Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L)
Braz. arch. biol. technol
;
62: e19180228, 2019. tab
Artigo
em Inglês
| LILACS
| ID: biblio-1019546
ABSTRACT
Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars within 8 weeks. The somatic embryos developed into rooted plants with a 100% survival rate upon transfer to the greenhouse.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Reguladores de Crescimento de Plantas
/
Sementes
/
Nitrato de Prata
/
Coffea
Tipo de estudo:
Estudos de avaliação
Idioma:
Inglês
Revista:
Braz. arch. biol. technol
Assunto da revista:
Biologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Costa Rica
Instituição/País de afiliação:
Agronomic Institute of Paraná/BR
/
University of Costa Rica/CR
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