Human Cytomegalovirus DNA Quantification and Gene Expression in Gliomas of Different Grades
Plos one
;
11(7)2016.
Artigo
em Inglês
| LILACS, SES-SP
| ID: biblio-1024785
ABSTRACT
Gliomas are the most common type of primary brain tumors. The most aggressive type, Glioblastoma multiforme (GBM), is one of the deadliest human diseases, with an average survival at diagnosis of about 1 year. Previous evidence suggests a link between human cytomegalovirus (HCMV) and gliomas. HCMV has been shown to be present in these tumors and several viral proteins can have oncogenic properties in glioma cells. Here we have investigated the presence of HCMV DNA, RNA and proteins in fifty-two gliomas of different grades of malignancy. The UL83 viral region, the early beta 2.7 RNA and viral protein were detected in 73%, 36% and 57% by qPCR, ISH and IHC, respectively. Positivity of the viral targets and viral load was independent of tumor type or grade suggesting no correlation between viral presence and tumor progression. Our results demonstrate high prevalence of the virus in gliomas from Brazilian patients, contributing to a better understanding of the association between HCMV infection and gliomas worldwide and supporting further investigations of the virus oncomodulatory properties.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Proteínas Virais
/
Brasil
/
Humanos
/
RNA Viral
/
Imuno-Histoquímica
/
Hibridização In Situ
/
Infecções por Citomegalovirus
/
Carga Viral
/
Citomegalovirus
/
Glioma
Tipo de estudo:
Fatores de risco
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Plos one
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
Secretaria da Saúde do Estado de São Paulo/BR
/
Universidade Federal do ABC/BR
/
Universidade de São Paulo/BR
Similares
MEDLINE
...
LILACS
LIS