A cell-culturing system for the study of interaction between macrophages infected with leishmania amazonensis
Rev. patol. trop
;
48(2): 99-108, 2019.
Artigo
em Inglês
| LILACS
| ID: biblio-1025792
ABSTRACT
The cell culture insert system is a culturing system for the study of contact-independent cellular communication. Leishmaniasis is a neglect tropical disease with no vaccines and the available drugs present toxic side effects. Studies on Leishmania interaction with host macrophages aim to develop strategies for parasite control and drug development. The purpose of this study was to evaluate the effects of interaction between non-infected and L. amazonensis-infected human macrophages, by using the cell culture system. The results showed that the infection index was reduced by 56.2% as compared to controls only when infected macrophages were inserted on both sides of the Transwell membranes. An improvement in macrophage viability was also observed in this cell culture. The levels of interleukin-1ß, an inflammatory cytokine, and nitric oxide, a microbicidal molecule, did not increase in L. amazonensis-infected macrophage cultures in the Transwell system; thus other soluble factors were responsible for parasite control
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Leishmania mexicana
/
Leishmaniose
/
Cultura Primária de Células
/
Macrófagos
Idioma:
Inglês
Revista:
Rev. patol. trop
Assunto da revista:
Medicina Tropical
/
Patologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual de Campinas/BR
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