Splenic ischemic preconditioning attenuates oxidative stress induced by hepatic ischemia-reperfusion in rats
Acta cir. bras
;
34(7): e201900707, 2019. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1038118
ABSTRACT
Abstract Purpose:
To evaluate the effects of splenic ischemic preconditioning (sIPC) on oxidative stress induced by hepatic ischemia-reperfusion in rats.Methods:
Fifteen male Wistar rats were equally divided into 3 groups SHAM, IRI and sIPC. Animals from IRI group were subjected to 45 minutes of partial liver ischemia (70%). In the sIPC group, splenic artery was clamped in 2 cycles of 5 min of ischemia and 5 min of reperfusion (20 min total) prior to hepatic ischemia. SHAM group underwent the same surgical procedures as in the remaining groups, but no liver ischemia or sIPC were induced. After 1h, hepatic and splenic tissue samples were harvested for TBARS, CAT, GPx and GSH-Rd measurement.Results:
sIPC treatment significantly decreased both hepatic and splenic levels of TBARS when compared to IRI group (p<0.01). Furthermore, the hepatic and splenic activities of CAT, GPx and GSH- Rd were significantly higher in sIPC group than in IRI group.Conclusion:
sIPC was able to attenuate hepatic and splenic IRI-induced oxidative stress.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Traumatismo por Reperfusão
/
Estresse Oxidativo
/
Precondicionamento Isquêmico
/
Fígado
/
Hepatopatias
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
UEPA/BR
/
Universidade do Estado do Pará/BR
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