Graves' ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
Arch. endocrinol. metab. (Online)
;
62(3): 366-369, May-June 2018. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1038490
ABSTRACT
ABSTRACT Objective:
Graves' ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials andmethods:
Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). rRARα gene expression in the treated and untreated cells was then compared.Results:
Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05).Conclusions:
Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Órbita
/
Dexametasona
/
Expressão Gênica
/
Oftalmopatia de Graves
/
Fibroblastos
/
Glucocorticoides
Limite:
Humanos
Idioma:
Inglês
Revista:
Arch. endocrinol. metab. (Online)
Assunto da revista:
Endocrinologia
/
Metabolismo
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Instituto Federal de Educação, Ciência e Tecnologia do Estado de São Paulo/BR
/
Universidade Estadual de São Paulo/BR
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