Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)
Biol. Res
;
51: 42, 2018. tab
Artigo
em Inglês
| LILACS
| ID: biblio-1038782
ABSTRACT
BACKGROUND: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. RESULTS: Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. CONCLUSIONS: From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
DNA de Plantas
/
Repetições de Microssatélites
/
Gaultheria
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Chile
/
Equador
/
Singapura
Instituição/País de afiliação:
BioTECNOS Ltda/CL
/
Pontificia Universidad Católica de Chile/CL
/
Universidad Católica del Maule/CL
/
Universidad Técnica de Manabí/EC
/
Verdant BioScience Pte. Ltda/SG
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