Development of an Extraction Protocol for Fetal Sexing from the Fourth Week of Gestation by Real Time PCR
Braz. arch. biol. technol
;
62: e19180382, 2019. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1039119
ABSTRACT
Abstract Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and clinical applications. This research developed a technology which allowed the extraction of fetal DNA by a non-invasive method that offers no risk to the mother or fetus. A total of 132 pregnant women participated in this inquiry. The DNA extraction was performed employing an in-house method based on guanidine thiocyanate and magnetic bead. For the amplification it was used the Quantifier Y Kit™. The fetal sexing analysis of the 132 pregnant women were 100% in agreement with the ultrasound. Sensitivity and specificity for detection of Y chromosome sequences was possible using Real-Time Polymerase Chain Reaction since the 4th week of gestation. This non-invasive early determination could be employed in fetal gender and also to be extended to detection of genetic diseases in the shortest possible time avoiding invasive methods that puts the fetus at risk.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Análise para Determinação do Sexo
/
Reação em Cadeia da Polimerase em Tempo Real
/
Teste Pré-Natal não Invasivo
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
Idioma:
Inglês
Revista:
Braz. arch. biol. technol
Assunto da revista:
Biologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of Mato Grosso do Sul/BR
/
Institute of Scientific Expertise Campo Grande/BR
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