Genetic analysis of the first mcr-1 positive Escherichia coli isolate collected from an outpatient in Chile
Braz. j. infect. dis
;
23(3): 203-206, May-June 2019. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1039226
ABSTRACT
ABSTRACT Global dissemination of mcr-like genes represents a serious threat to public health since it jeopardizes the effectiveness of colistin, an antibiotic used as a last-resort treatment against highly antibiotic-resistant bacteria. In 2017, a mcr-1-positive isolate of Escherichia coli was found in Chile for the first time. Herein we report the genetic features of this strain (UCO-457) by whole-genome sequencing (WGS) and conjugation experiments. The UCO-457 strain belonged to ST4204 and carried a 285 kb IncI2-type plasmid containing the mcr-1 gene. Moreover, this plasmid was transferred by conjugation to an E. coli J53 strain at high frequency. The isolate harbored the cma, iroN, and iss virulence genes and did carry resistance genes to trimethoprim/sulfamethoxazole and fluoroquinolones. Other antibiotic resistance determinants such as β-lactamases-encoding genes were not detected, making the isolate highly susceptible to these antibiotics. Our results revealed that such susceptible isolates could be acting as platforms to disseminate plasmid-mediated colistin resistance. Based on this evidence, we consider that mcr-like prevalence deserves urgent attention and should be examined not only in highly resistant bacteria but also in susceptible isolates.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Proteínas de Escherichia coli
/
Escherichia coli
/
Infecções por Escherichia coli
Tipo de estudo:
Fatores de risco
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Chile
Idioma:
Inglês
Revista:
Braz. j. infect. dis
Assunto da revista:
Doenças Transmissíveis
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
Pontificia Universidad Católica de Chile/CL
/
Universidad de Concepción/CL
/
Universidad del Bio-Bio/CL
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