A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
Mem. Inst. Oswaldo Cruz
;
114: e190158, 2019. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1040629
ABSTRACT
As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N- and C-terminus of P. vivax Merozoite Surface Protein-1 (Nterm-PvMSP1 and MSP119). The resulting assay is simple and efficient for use as a routine phagocytic assay for the evaluation of merozoite stage vaccine candidates.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fagocitose
/
Plasmodium vivax
/
Anticorpos Antiprotozoários
/
Proteínas de Protozoários
/
Merozoítos
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fundação Oswaldo Cruz-Fiocruz/BR
/
Fundação de Medicina Tropical Dr Heitor Vieira Dourado/BR
/
Universidade Federal do Amazonas/BR
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