Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Rev. Soc. Bras. Med. Trop
;
51(1): 66-70, Jan.-Feb. 2018. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1041442
ABSTRACT
Abstract INTRODUCTION: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. METHODS: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. RESULTS: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. CONCLUSIONS: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Replicação Viral
/
Vírus da Febre Amarela
/
Montagem de Vírus
/
Vacinas de Partículas Semelhantes a Vírus
Limite:
Humanos
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Estados Unidos
Instituição/País de afiliação:
Fundação Oswaldo Cruz/BR
/
Universidade Federal do Pará/BR
/
University of Georgia/US
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