Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
Rev. Soc. Bras. Med. Trop
;
51(3): 387-389, Apr.-June 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1041461
ABSTRACT
Abstract INTRODUCTION:
Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated.METHODS:
We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA).RESULTS:
The conidia concentration required for an EIA-positive result was 4.8 × 103 (median).CONCLUSIONS:
This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Aspergillus fumigatus
/
Esporos Fúngicos
/
Ensaio de Imunoadsorção Enzimática
/
Reações Falso-Positivas
/
Mananas
Limite:
Humanos
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de Pelotas/BR
/
Universidade Federal do Rio Grande/BR
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