IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase
Rev. Soc. Bras. Med. Trop
;
52: e20180139, 2019. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1041506
ABSTRACT
Abstract INTRODUCTION:
High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins.METHODS:
Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.RESULTS:
Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.CONCLUSIONS:
Potato apyrase and SmATPDases have IgE-binding epitopes.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Apirase
/
Schistosoma mansoni
/
Esquistossomose mansoni
/
Solanum tuberosum
/
Imunoglobulina E
/
Anticorpos Anti-Helmínticos
/
Epitopos
Limite:
Animais
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fundação Oswaldo Cruz/BR
/
Universidade Federal de Juiz de Fora/BR
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