Comparison of methodologies for detecting Trypanosoma cruzi parasites by microscopic observation of microhematocrit capillary tubes
Rev. Soc. Bras. Med. Trop
;
52: e20180505, 2019. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1041570
ABSTRACT
Abstract INTRODUCTION: The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood. METHODS: The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity. RESULTS: The rotating method was easier, faster, and more sensitive than the others with 100% specificity. CONCLUSIONS: The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Trypanosoma cruzi
/
Centrifugação
/
Doença de Chagas
/
Parasitemia
/
Tubo Capilar
/
Hematócrito
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Bélgica
/
México
Instituição/País de afiliação:
Universidad Autónoma de Yucatán/MX
/
Université Libre de Bruxelles/BE
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