Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein
Electron. j. biotechnol
;
39: 52-60, may. 2019. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1052027
ABSTRACT
BACKGROUND: Biologically active peptides produced from fish wastes are gaining attention because their health benefits. Proteases produced by halophilic microorganisms are considered as a source of active enzymes in high salt systems like fish residues. Hence, the aim of this study was the bioprospection of halophilic microorganisms for the production of proteases to prove their application for peptide production. RESULTS: Halophilic microorganisms were isolated from saline soils of Mexico and Bolivia. An enzymatic screening was carried out for the detection of lipases, esterases, pHB depolymerases, chitinases, and proteases. Most of the strains were able to produce lipases, esterases, and proteases, and larger hydrolysis halos were detected for protease activity. Halobacillus andaensis was selected to be studied for proteolytic activity production; the microorganism was able to grow on gelatin, yeast extract, skim milk, casein, peptone, fish muscle (Cyprinus carpio), and soy flour as protein sources, and among these sources, fish muscle protein was the best inducer of proteolytic activity, achieving a protease production of 571 U/mL. The extracellular protease was active at 50°C, pH 8, and 1.4 M NaCl and was inhibited by phenylmethylsulfonyl fluoride. The proteolytic activity of H. andaensis was used to hydrolyze fish muscle protein for peptide production. The peptides obtained showed a MW of 5.3 kDa and a radical scavenging ability of 10 to 30% on 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and a ferric reducing ability of plasma. Conclusion: The use of noncommercial extracellular protease produced by H. andaensis for biologically active peptide production using fish muscle as the protein source presents a great opportunity for high-value peptide production.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Peptídeo Hidrolases
/
Peptídeos
/
Proteínas de Peixes
/
Halobacillus
País/Região como assunto:
América do Sul
/
Bolívia
/
México
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
México
Instituição/País de afiliação:
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C/MX
/
Universidad Autónoma de Coahuila/MX
/
Universidade Federal do Paraná/BR
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