Productive infection and transduction by bacteriophage P1 in the species Salmonella bongori
Electron. j. biotechnol
;
41: 9-12, sept. 2019. tab, ilus
Artigo
em Inglês
| LILACS
| ID: biblio-1053556
ABSTRACT
Background: Horizontal gene transfer (HGT) is the most important mechanism in the evolution of new genetic capabilities in bacteria, including specific degradative pathways, virulence factors, and resistance to antibiotics. Among the processes involved in HGT, transduction is noteworthy. This is a mechanism for gene transmission mediated by a bacteriophage that functions both as a reservoir and as a vector of exogenous genes, which remain protected from environmental effects in the bacteriophage's capsid. Within this context, this investigation aimed to evaluate the ability of the generalized transducing bacteriophage P1 to productively infect and transduce in the bacterial species Salmonella bongori. Results: We could establish that a derivative of bacteriophage P1, P1Cm, infects strains of S. bongori with frequencies of lysogenization in the order of ~10−2 lysogens/UFP. Through thermal induction, infective viral progeny was obtained, and we could show that P1Cm readily formed plaques on S. bongori lawns, a phenomenon thus far not reported for other members of the genus Salmonella. Finally, we showed P1Cm-mediated transduction of the model plasmid RP4 at frequencies of ~10−7 transductants/donor. Conclusion: Therefore, bacteriophage P1 can be used as a tool for the genetic manipulation in the species S. bongori.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Salmonella
/
Transdução Genética
/
Bacteriófago P1
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Canadá
/
Chile
Instituição/País de afiliação:
Memorial University of Newfoundland/CA
/
Pontificia Universidad Católica de Valparaíso/CL
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