Co-transfection of hepatocyte growth factor and truncated TGF-ß type II receptor inhibit scar formation
Braz. j. med. biol. res
;
53(1): e9144, Jan. 2020. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1055480
ABSTRACT
Wound scarring remains a major challenge for plastic surgeons. Transforming growth factor (TGF)-β plays a key role in the process of scar formation. Previous studies have demonstrated that truncated TGF-β type II receptor (t-TGF-βRII) is unable to continue signal transduction but is still capable of binding to TGF-β, thereby blocking the TGF-β signaling pathway. Hepatocyte growth factor (HGF) is a multifunctional growth factor that promotes tissue regeneration and wound healing. Theoretically, the combination of HGF and t-TGF-βRII would be expected to exert a synergistic effect on promoting wound healing and reducing collagen formation. In the present study, lentivirus-mediated transfection of the two genes (t-TGF-βRII/HGF) into fibroblasts in vitro and in a rat model in vivo was used. The results demonstrated that the expression of t-TGF-βRII and HGF in NIH-3T3 cells was successfully induced. The expression of both molecules significantly reduced collagen I and III expression, and also inhibited fibroblast proliferation. Furthermore, histological examination and scar quantification revealed less scarring in the experimental wound in a rat model. Moreover, on macroscopic inspection, the experimental wound exhibited less visible scarring compared with the control. Therefore, the present study demonstrated that the combination gene therapy of t-TGF-βRII and HGF promoted wound healing, with less scarring and more epithelial tissue formation, not only by suppressing the overgrowth of collagen due to its antifibrotic effect, but also by promoting tissue regeneration.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Transfecção
/
Colágeno
/
Cicatriz
/
Fator de Crescimento de Hepatócito
/
Fator de Crescimento Transformador beta2
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
First Affiliated Hospital, Zhejiang University School of Medicine/CN
/
Sir Run Run Shaw Hospital, Zhejiang University School of Medicine/CN
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