Learning from transgenics: advanced gene editing technologies should also bridge the gap with traditional genetic selection
Electron. j. biotechnol
;
41: 22-29, sept. 2019. ilus
Artigo
em Inglês
| LILACS
| ID: biblio-1087255
ABSTRACT
We highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants, transgenic or genome editing (CRISPR/Cas9). The incidence of undesirable somaclonal variations and/or epigenetic changes that might have occurred during in vitro multiplication and regeneration processes, must be carefully analyzed in replicates in field trials. One remarkable challenge is related to the time lapse that selects the modified elite genotypes, because these strategies may spend a variable amount of time before the results are commercialized, where in all the cases it should be take into account the genotypeâ¯×â¯environment interactions. Furthermore, this combination of techniques can create an encouraging bridge between the public opinion and the community of geneticists who are concerned with plant genetic improvement. In this context, either transgenesis or genomic editing strategies become complementary modern tools to facing the challenges of plant genetic improvement. Their applications will depend on case-by-case analysis, and when possible will necessary associate them to the schemes and bases of classic plant genetic improvement.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Plantas Geneticamente Modificadas
/
Técnicas de Transferência de Genes
/
Sistemas CRISPR-Cas
/
Edição de Genes
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Chile
/
Itália
Instituição/País de afiliação:
Universidad Católica del Maule/CL
/
Universidade Federal de São Carlos/BR
/
Universitá di Salerno/IT
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