Putative periodontal bacteria in clinically healthy and diseased sites of periodontitis patients
Braz. j. oral sci
;
18: e191417, jan.-dez. 2019. ilus
Artigo
em Inglês
| LILACS, BBO
| ID: biblio-1095042
ABSTRACT
Aim:
The aim of this study was to compare the microbial profile of subgingival sites in Periodontitis (Pd) patients and healthy ones.Methods:
Eighteen patients with Pd and 18 gender-matched healthy controls were selected. Subgingival samples were collected from three types of sites 1) healthy site of healthy subjects (probing pocket depth (PPD) ≤ 3mm, CG), 2) healthy site of Pd patients (PPD ≤ 3mm, PG-C) and 3) diseased site (PPD > 3mm) of the same Pd patients (PG-T). All sites were subjected to microbial analysis for the detection of 40 bacterial species by the "Checkerboard DNA-DNA hybridization" technique.Results:
It was observed a great diversity of bacteria in all patients evaluated. The sites from the Pd groups (PG-T and PG-C) showed a higher overall count of the studied bacteria than those of the CG group, especially from Green, Orange, and Red complexes. Also, PG-T showed a higher prevalence of Red complex bacteria than CG. Individual pathogens, such as Porphyromonas gingivalis, Treponema denticola and Treponema socranskii were detected in higher levels and/or prevalence in Pd than in control patients. However, it was not observed any difference between PG-T and PG-C.Conclusion:
Pd patients showed higher prevalence and counts of some putative periodontal bacteria, especially from the red complex, than control ones, regardless of the severity of their sites
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Periodontite
/
Bactérias
/
Biologia Molecular
Tipo de estudo:
Fatores de risco
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. j. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Veiga de Almeida University (UVA)/BR
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