Detection of salivirus in raw sewage samples in Rio de Janeiro, Brazil
Rev. patol. trop
;
49(2)2020.
Artigo
em Inglês
| LILACS
| ID: biblio-1100644
ABSTRACT
Gastroenteric viruses are important pathogens related to cases of acute gastroenteritis, affecting millions of people worldwide with a major impact on children under five in developing countries. The introduction of metagenomic approach techniques in the 2000s has allowed the description of new viruses, among them Salivirus, which has been associated worldwide with cases of diarrhea. This study aimed to detect salivirus in raw sewage samples from a wastewater treatment plant (WWTP) collected between June 2013 and May 2014 in Rio de Janeiro, Brazil. Fifty-two samples collected weekly were tested by using a real-time quantitative PCR (qPCR). Salivirus genome was detected in 71.1% (37/52) of the samples, with viral concentration ranging from 7.56 x 104 to 7.20 x 106 genomic copies per liter. Higher viral loads were detected in the summer and fall of 2014, although these data were not sufficient to infer seasonality for this virus. The high prevalence of salivirus in sewage samples highlights the importance of viral research in wastewater to generate data on salivirus circulation, increasing understanding regarding its dissemination in the population.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Esgotos
/
Reação em Cadeia da Polimerase em Tempo Real
/
Gastroenterite
Tipo de estudo:
Estudo diagnóstico
/
Fatores de risco
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Rev. patol. trop
Assunto da revista:
Medicina Tropical
/
Patologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fundação Oswaldo Cruz/BR
/
Universidade Federal de Goiás/BR
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