Resistencia a nitrofuranos en Salmonella enterica aisladas de carne para consumo humano / Nitrofuran resistance in Salmonella enterica isolated from meat for human consumption
Rev. peru. med. exp. salud publica
;
37(1): 99-103, ene.-mar. 2020. tab
Artigo
em Espanhol
| LILACS
| ID: biblio-1101813
RESUMEN
RESUMEN En el presente estudio, se analizaron los mecanismos de resistencia a nitrofuranos en 18 muestras cár nicas con Salmonella enterica (15 de pollo, 2 de ternera y 1 de cerdo) de mercados de Lima (Perú). Determinaron los serotipos de los aislamientos y la sensibilidad a furazolidona y nitrofurantoina (con y sin el inhibidor de bombas de expulsión Phenyl-Arginine-β-Naphthylamide [PAβN]), las mutaciones en los genes snrA y cnr por PCR y la transferabilidad de la resistencia por conjugación. Se identificaron 15 muestras con S. infantis (13 muestras de pollo), 2 con S. enteritidis y 1 con S. anatum. Todos los aisla mientos, excepto S. anatum, fueron resistentes a ambos nitrofuranos (concentración mínima inhibidora [CMI] a furazolidona: 32-64 µg/mL, CMI a nitrofurantoina: 128-256 µg/mL), sin diferencias al adicio narse PAβN. Todos los aislamientos resistentes a nitrofuranos presentaron sustituciones en snrA y cnr (S. infantis: snrA STOP-151; cnr STOP-137; S. enteritidis: snrA STOP-180; cnr STOP-179). No se detectaron mecanismos transferibles de resistencia a nitrofuranos.
ABSTRACT
ABSTRACT The mechanisms of resistance to nitrofurans from 18 meat samples with Salmonella enterica (chicken: 15; beef: 2; pork: 1) collected in Lima (Peru) were analyzed. The isolates were serotyped and the susceptibility levels to furazolidone and nitrofurantoin [with and without the efflux pump inhibitor Phenyl-Arginine- β-naphthylamide (PAβN)], the presence of mutations in the snrA and cnr genes and the transferability of resistance by conjugation were established. Fifteen samples with S. infantis (13 from chicken samples), 2 with S. enteritidis and 1 with S. anatum were identified. All isolates except the S. anatum were resistant to both nitrofurans showing MICs (minimum inhibitory concentration) of furazolidone and nitrofurantoin of 32-64 μg/mL and 128-256 μg/mL, respectively. The addition of PAßN had no effect on the MIC levels. All nitrofuran-resistant isolates showed amino acid codon alterations at both snrA and cnr (S. infantis: snrA STOP-151; cnr STOP-137; S. enteritidis: snrA STOP-180; cnr STOP-179). No transferable mecha nisms of nitrofuran resistance were detected.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Salmonella enterica
/
Farmacorresistência Bacteriana
/
Microbiologia de Alimentos
/
Carne
/
Nitrofurantoína
Limite:
Animais
/
Humanos
País/Região como assunto:
América do Sul
/
Peru
Idioma:
Espanhol
Revista:
Rev. peru. med. exp. salud publica
Assunto da revista:
Saúde Pública
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Peru
/
Espanha
Instituição/País de afiliação:
Nutreco/ES
/
Universidad Científica del Sur/PE
/
Universidad Peruana Cayetano Heredia/PE
/
Universitat de Barcelona/ES
Similares
MEDLINE
...
LILACS
LIS