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miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
Degerli, Elif; Torun, Vildan; Cansaran-Duman, Demet.
  • Degerli, Elif; Ankara University. Biotechnology Institute. Ankara. TR
  • Torun, Vildan; Ankara University. Biotechnology Institute. Ankara. TR
  • Cansaran-Duman, Demet; Ankara University. Biotechnology Institute. Ankara. TR
Biol. Res ; 53: 19, 2020. graf
Artigo em Inglês | LILACS | ID: biblio-1114696
ABSTRACT

BACKGROUND:

Breast cancer is the most common cancer types among women. Recent researches have focused on determining the efficiency of alternative molecules and miRNAs in breast cancer treatment. The AIMof this study was to determine the effect of usnic acid response-miR-185-5p on proliferation in the breast cancer cell and to determine its relationship with apoptosis pathway.

METHODS:

The cell proliferation and cell apoptosis rate were significantly increased following the ectopic expression of miR-185-5p in BT-474 cells. Furthermore, the results of cell cycle assay performed by flow cytometry revealed that the transfection with miR-185-5p induced G1/S phase arrest. The apoptosis-related genes expression analysis was performed by qRT-PCR and the direct target of miR-185-5p in BT-474 cells was identified by western blot and luciferase reporter assay.

RESULTS:

Our data showed that miR-185-5p can cause significant changes in apoptosis-related genes expression levels, suggesting that cell proliferation was suppressed by miR-185-5p via inducing apoptosis in breast cancer cells. According to western blot results, miR-185-5p lead to decrease BCL2 protein level in BT-474 cells and direct target of miR-185-5p was identified as BCL by luciferase reporter assay.

CONCLUSION:

This study revealed that miR-185-5p may be an effective agent in the treatment of breast cancer.
Assuntos


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Benzofuranos / Neoplasias da Mama / Proteínas Proto-Oncogênicas c-bcl-2 / MicroRNAs Limite: Feminino / Humanos Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: Turquia Instituição/País de afiliação: Ankara University/TR

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Benzofuranos / Neoplasias da Mama / Proteínas Proto-Oncogênicas c-bcl-2 / MicroRNAs Limite: Feminino / Humanos Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: Turquia Instituição/País de afiliação: Ankara University/TR