Antifungal activity of the essential oil of cymbopogon citratus (DC) stapf: an in vitro study
J. oral res. (Impresa)
;
6(12): 319-323, dic. 30, 2017. tab
Artigo
em Inglês
| LILACS
| ID: biblio-1118785
ABSTRACT
Aim. to evaluate the antifungal potential of the essential oil of cymbopogon citratus by determining the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) for candida albicans (ATCC 90029), candida albicans (CBS 562), candida tropicalis (ATCC 705) and candida tropicalis strains (CBS 94), as well as to analyze the possible mechanism of action of the oil through the addition of sorbitol to the culture medium. methods. for the MIC determination, inocula were previously adjusted through spectrophotometry and 100µL were added to the wells of plates already containing the culture medium and 100µL of the serial dilutions of the oil, incubating them in aerobiosis for 24 hours, with subsequent staining by 1 percent TCT. for the MFC, 50µL of the supernatant from the MIC assay wells were dripped onto petri dishes and incubated in aerobiosis for 24 hours. tests were performed in triplicate and data analysed by descriptive statistics. results. It was determined that the MIC for C. albicans was 125 µg/mL while MIC for C. tropicalis was 250 µg/mL, with the essential oil presenting fungicidal effect for both analyzed yeasts. conclusion. the essential oil of cymbopogon citratus does not act at the cellular wall level and demonstrated an antimicrobial effect on candida albicans and candida tropicalis, therefore acting as a fungicide.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Candida albicans
/
Óleos Voláteis
/
Extratos Vegetais
/
Candida tropicalis
/
Cymbopogon
/
Antifúngicos
Limite:
Humanos
Idioma:
Inglês
Revista:
J. oral res. (Impresa)
Assunto da revista:
Odontologia
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of Paraiba/BR
/
State University of Paraiba/BR
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