Loop-mediated isothermal amplification (LAMP) assay for rapid and accurate confirmatory diagnosis of HTLV-1/2 infection
Viruses
;
12(981): 1-15, 2020. ilus
Artigo
em Inglês
| LILACS, SES-SP, SESSP-IALPROD, SES-SP
| ID: biblio-1122302
ABSTRACT
Laboratory diagnosis of human T-lymphotropic viruses (HTLV) 1 and 2 infection is performed by serological screening and further confirmation with serological or molecular assays. Thus, we developed a loop-mediated isothermal nucleic acid amplification (LAMP) assay for the detection of HTLV-1/2 in blood samples. The sensitivity and accuracy of HTLV-1/2 LAMP were defined with DNA samples from individuals infected with HTLV-1 (n = 125), HTLV-2 (n = 19), and coinfected with HIV (n = 82), and compared with real-time polymerase chain reaction (qPCR) and PCR-restriction fragment length polymorphism (RFLP). The overall accuracy of HTLV-1/2 LAMP (95% CI 74.885.5%) was slightly superior to qPCR (95% CI 69.581.1%) and similar to PCR-RFLP (95% CI 79.589.3%). The sensitivity of LAMP was greater for HTLV-1 (95% CI 83.293.4%) than for HTLV-2 (95% CI 43.270.8%). This was also observed in qPCR and PCR-RFLP, which was associated with the commonly lower HTLV-2 proviral load. All molecular assays tested showed better results with samples from HTLV-1/2 mono-infected individuals compared with HIV-coinfected patients, who present lower CD4 T-cell counts. In conclusion, HTLV-1/2 LAMP had similar to superior performance than PCR-based assays, and therefore may represent an attractive alternative for HTLV-1/2 diagnosis due to reduced working time and costs, and the simple infrastructure needed.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Vírus
/
DNA
/
Vírus Linfotrópico T Tipo 1 Humano
/
Vírus Linfotrópico T Tipo 2 Humano
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Viruses
Ano de publicação:
2020
Tipo de documento:
Artigo
Instituição/País de afiliação:
Fiocruz/BR
/
Secretaria da Saúde do Estado de São Paulo/BR
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