Assessment of Experimental Models for Obtaining Adipose-Derived Mesenchymal Stem Cells
Braz. arch. biol. technol
;
63: e20190260, 2020. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1132251
ABSTRACT
Abstract Creating experimental models for obtaining stem cells from adipose tissue is necessary to elucidate their peculiar features. Objective:
This study proposed a reliable reproducible and consistent experimental model for obtaining mesenchymal stem cells from adipose tissue. Material andMethod:
Lines of New Zealand rabbits, Wistar rats and CaviaPorcellus guinea pigs (4 animals per species) were used. Fatty tissue mesenchymal stem cells were removed from dorsal, epididymal and inguinal regions. Percentage viable cells and percentage cells expanded and submitted to chondrogenic differentiation were compared by animal species and collection site.Results:
Chondrogenic differentiation occurred in a similar manner across all samples, independently of animal species or collection site. Among samples assessed, the inguinal region of rats yielded the highest percentage of viable and expanded cells.Conclusion:
A reliable, reproducible and consistent model for obtaining mesenchymal stem cells was produced. Of the several variables analysed, the best results were obtained from the inguinal region of the rat.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Tecido Adiposo
/
Células-Tronco Mesenquimais
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. arch. biol. technol
Assunto da revista:
Biologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of ABC/BR
/
Santa Casa de São Paulo/BR
/
State University of Ponta Grossa/BR
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