Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina
Braz. arch. biol. technol
;
63: e20190272, 2020. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1132258
ABSTRACT
Abstract The Apple Germplasm Bank (AGB) of Santa Catarina Agricultural Research and Rural Extension Company - Epagri, AGB-Epagri, is the largest of the genus Malus in Brazil. Twenty-eight main accessions of this bank were virus screened through DAS-ELISA, RT-PCR and IC-RT-PCR during two consecutive reproductive cycles, and each accession showed latent mixed infection by at least two species, among ASGV, ASPV and ACLSV. The combined use of diagnostic methods helped overcome inconsistencies commonly found in apple virus detection and was shown essential for the AGB-Epagri can be safely used as a source of genetic variability and for the exchange of virus-free propagative material.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Malus
/
Flexiviridae
/
Banco de Sementes
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Braz. arch. biol. technol
Assunto da revista:
Biologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Embrapa Grape and Wine/BR
/
Epagri/BR
/
State University of Maringá/BR
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