Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund's adjuvant-induced rheumatoid arthritis via inhibition of NF-κB
Braz. j. med. biol. res
;
53(6): e9489, 2020. graf
Artigo
em Inglês
| LILACS, ColecionaSUS
| ID: biblio-1132521
ABSTRACT
Rheumatoid arthritis (RA) is an autoimmune disease of knee joints involving pain and inflammation. Rhoifolin is a plant flavonoid known to have antioxidant and anti-inflammatory properties. This study was taken to identify the effect of rhoifolin on complete Freund's adjuvant (CFA)-induced arthritis in the rat model. Treatment with rhoifolin (10 and 20 mg/kg) showed a significant improvement in the overall health parameters such as paw edema and weight loss. This improvement in morphological parameters corroborated the findings with gross morphological changes observed in the histopathological analysis. Rhoifolin treatment also caused a significant decrease in oxidative stress, evident from changes in intracellular levels of glutathione, glutathione peroxidase, malondialdehyde, and superoxide dismutase in the articular cartilage tissue. Moreover, proinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin(IL)-1β, and IL-6 showed a significant downregulation of gene expression and intracellular protein concentration levels. The NF-κB pathway showed a significant attenuation as evident in the significant reduction in the levels of NF-κB p65 and p-IκB-α. These results indicated that rhoifolin can be a natural therapeutic alternative to the extant regimens, which include non-steroidal anti-inflammatory drugs and immunosuppressants. Additionally, the antioxidant and anti-inflammatory action of rhoifolin was probably mediated by the NF-κB pathway. However, the exact target molecules of this pathway need to be determined in further studies.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Artrite Experimental
/
Artrite Reumatoide
/
Flavonoides
/
Adjuvante de Freund
/
Citocinas
/
Estresse Oxidativo
/
Dissacarídeos
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Ano de publicação:
2020
Tipo de documento:
Artigo
Instituição/País de afiliação:
Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine/CN
/
Guangzhou University of Chinese Medicine/CN
/
The 455th Hospital of Chinese Peoples Liberation Army/CN
/
Wenzhou Hospital of Chinese Medicine/CN
/
YouJiang Medical University for Nationalities/CN
Similares
MEDLINE
...
LILACS
LIS