In vitro-induction of metronidazole-resistant Giardia duodenalis is not associated with nucleotide alterations in the genes involved in pro-drug activation
Mem. Inst. Oswaldo Cruz
;
115: e200303, 2020. tab, graf
Artigo
em Inglês
| LILACS, SES-SP
| ID: biblio-1135270
ABSTRACT
Giardiasis is an infectious disease caused by Giardia duodenalis. The pro-drug metronidazole (MTZ) is the first-line treatment for giardiasis. Parasite's proteins as pyruvateferredoxin oxidoreductase (PFOR), ferredoxin (Fd), nitroreductase-1 (NR-1) and thioredoxin reductase (TrxR) participate in MTZ activation. Here, we showed Giardia trophozoites long-term exposed to MTZ presented higher IC50 than controls, showing the drug influenced the parasite survival. That reduction in MTZ's susceptibility does not seem to be related to mutations in the genes pfor, fd, nr-1 or trxr. It points that different mechanism as alterations in other metabolic pathways can account for Giardia resistance to MTZ therapy.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Resistência a Medicamentos
/
Pró-Fármacos
/
Giardia lamblia
/
Metronidazol
/
Antiprotozoários
Tipo de estudo:
Fatores de risco
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Ano de publicação:
2020
Tipo de documento:
Artigo
Instituição/País de afiliação:
Fundação Oswaldo Cruz-Fiocruz/BR
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