Risk Factors for Early Childhood Caries Based on Identification of Veillonella spp. Using RT-PCR
Pesqui. bras. odontopediatria clín. integr
;
20: e5701, 2020. tab
Artigo
em Inglês
| LILACS, BBO
| ID: biblio-1135530
ABSTRACT
Abstract Objective:
To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2-3 years. Material andMethods:
A cross-sectional survey was conducted and samples from 87 children aged 2-3 years, who lived in selected villages in the Bandung City area, Indonesia, were collected. Examination for dental caries was performed using standard checks for decay, missing, and filled surfaces (dfms), and saliva samples were taken. Microbiological examination was performed using RT-PCR with primers consisting of one primary set for Veillonella spp. and one universal primary set for 16S rDNA. We performed statistical testing using the Mann Whitney rank-sum test.Results:
A total of 87 children were sampled, and an ECC prevalence of 71.3% was found, with a mean dmfs of 7.1 (± 9.1). The proportion of Veillonella spp. in caries-free children was 2.13 ± 2.30, while in children with ECC, it was 3.29 ± 6.83.Conclusion:
The proportion of Veillonella spp. in children with ECC was higher than in caries-free children; therefore, Veillonella spp. may be a risk factor for ECC.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Veillonella
/
Reação em Cadeia da Polimerase
/
Fatores de Risco
/
Cárie Dentária
/
Bactérias Gram-Negativas
Tipo de estudo:
Estudo diagnóstico
/
Estudo de etiologia
/
Estudo observacional
/
Estudo de prevalência
/
Estudo prognóstico
/
Fatores de risco
Limite:
Criança, pré-escolar
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Pesqui. bras. odontopediatria clín. integr
Assunto da revista:
Odontologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Indonésia
Instituição/País de afiliação:
University of Indonesia/ID
/
University of Padjadjaran/ID
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