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miR-139-5p protects septic mice with acute lung injury by inhibiting Toll-like receptor 4/Myeloid differentiation factor 88/Nuclear factor-&mac_kgr; B signaling pathway
Zhang, Xiuxiu; Liu, Xin; Chang, Rui; Li, Yue.
  • Zhang, Xiuxiu; Eastern District of the Jining No.1 Peoples Hospital. Departments of Critical Care Medicine. Jining. CN
  • Liu, Xin; Eastern District of the Jining No.1 Peoples Hospital. Departments of Critical Care Medicine. Jining. CN
  • Chang, Rui; Eastern District of the Jining No.1 Peoples Hospital. Departments of Critical Care Medicine. Jining. CN
  • Li, Yue; Eastern District of the Jining No.1 Peoples Hospital. Departments of Emergency Critical Care Medicine. Jining. CN
Clinics ; 76: e2484, 2021. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1153996
ABSTRACT
OBJECTIVES: To investigate the role of miR-139-5p and the TLR4/MyD88/NF-κB signaling pathway in acute lung injury in septic mice. METHOD: A total of 140 healthy male SPF C57BL/6 mice were divided into seven groups, i.e., Normal, Control, NC, miR-139-5p mimic, miR-139-5p inhibitor, TAK-242, and miR-139-5p inhibitor+TAK-242 groups. The levels of miR-139-5p, proteins related to the TLR4/MyD88/NF-κB signaling pathway (TLR4, MyD88, and p-NF-κB p50), and MPO, SOD, GSH, and MDA in lung tissue were measured. The lung tissue wet-to-dry mass ratio (W/D), arterial oxygen partial pressure (PaO2), and carbon dioxide partial pressure (PaCO2) were measured. RESULTS: A web-based bioinformatic tool predicted that MyD88 was a target of miR-139-5p, which was verified by a dual luciferase reporter assay. Compared with those in the Normal group, the levels of miR-139-5p, PaO2, SOD, and GSH were significantly lower, while those of TLR4, MyD88, p-NF-κB p50, W/D, PaCO2, IL-1β, TNF-α, IL-6, MPO, and MDA were higher in all other groups. Moreover, compared with their levels in the Control group, these indicators exhibited contrasting results in the miR-139-5p mimic and TAK-242 groups, but were similar in the miR-139-5p inhibitor group. In the miR-139-5p inhibitor+TAK-242 group, acute lung injury, aggravated by miR-139-5p inhibitor, was partially rescued by TAK-242. CONCLUSION: miR-139-5p inhibits the TLR4/MyD88/NF-κB signaling pathway to alleviate acute lung injury in septic mice.
Assuntos


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Sepse / MicroRNAs / Lesão Pulmonar Aguda Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Clinics Assunto da revista: Medicina Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Eastern District of the Jining No.1 Peoples Hospital/CN

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Sepse / MicroRNAs / Lesão Pulmonar Aguda Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Clinics Assunto da revista: Medicina Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Eastern District of the Jining No.1 Peoples Hospital/CN