Dengue diagnostics: serious inaccuracies are likely to occur if pre-analytical conditions are not strictly followed
Mem. Inst. Oswaldo Cruz
;
115: e200287, 2020. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1154869
ABSTRACT
BACKGROUND The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting. OBJECTIVES To check if pre-analytical variables, such as storage time and temperature, have an impact on the accuracy of the main routine diagnostic tests for dengue. METHODS Virus isolation, reverse transcription real-time polymerase chain reaction (RT-PCR) and NS1 enzyme-linked immunosorbent assay (ELISA) were evaluated using 84 samples submitted to different pre-analytical conditions. FINDINGS Sensitivity and negative predictive value were directly affected by sample storage conditions. RT-PCR and virus isolation showed greater dependence on well-conserved samples for an accurate diagnosis. Interestingly, even storage at -30ºC for a relatively short time (15 days) was not adequate for accurate results using virus isolation and RT-PCR tests. On the other hand, NS1 ELISA showed no significant reduction in positivity for aliquots tested under the same conditions as in the previous tests. MAIN CONCLUSIONS Our results support the stability of the NS1 marker in ELISA diagnosis and indicate that the accuracy of routine tests such as virus isolation and RT-PCR is significantly affected by inadequate transport and storage conditions of serum samples.
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Índice:
LILACS (Américas)
Assunto principal:
Testes Imunológicos
/
Ensaio de Imunoadsorção Enzimática
/
Proteínas não Estruturais Virais
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Dengue
/
Vírus da Dengue
/
Antígenos Virais
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Centro Universitário de Belo Horizonte/BR
/
Fundação Ezequiel Dias/BR
/
Universidade Federal de Minas Gerais/BR
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