Gastric mucosal protection by acetazolamide in rats. Roles of prostaglandins, sulfhydryls, and gastric motility

ABSTRACT

The aim of this study was to test the hypothesis that protective effect of subcutaneous acetazolamide, a carbonic anhydrase inhibitor, against ethanol-induced gastric mucosal damage is dependent on indomethacin- or iodoacetamide-sensitive mechanisms. In addition we studied the effects of acetazolamide on gastric motility and the influence of indomethacin and iodoacetamide on this parameter. Indomethacin (30 mg/kg) or iodoacetamide (100 ag/kg) was administered subcutaneously in doses that previously had been demonstrated to inhibit endogenous prostaglandins synthesis and gastric mucosal sulfhydryls respectively. At 30 min after these or control subcutaneous pretreatment, the rats were given subcutaneous acetazolamide or vehicle. Thirty min later 96