Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G> A with Orofacial Cleft
Pesqui. bras. odontopediatria clín. integr
;
21: e5896, 2021. tab, graf
Artigo
em Inglês
| LILACS, BBO
| ID: biblio-1180869
ABSTRACT
ABSTRACT Objective:
To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material andMethods:
A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test.Results:
The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group.Conclusion:
The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Polimorfismo Genético
/
Variação Genética
/
Fenda Labial
/
Fissura Palatina
/
Metilenotetra-Hidrofolato Desidrogenase (NADP)
Tipo de estudo:
Estudos de avaliação
/
Fatores de risco
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Pesqui. bras. odontopediatria clín. integr
Assunto da revista:
Odontologia
Ano de publicação:
2021
Tipo de documento:
Artigo
País de afiliação:
Indonésia
Instituição/País de afiliação:
Universitas Indonesia/ID
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