Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli
Electron. j. biotechnol
;
46: 8-13, jul. 2020. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1223212
ABSTRACT
BACKGROUND:
Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to be one of critical factors to lower the production cost of PHB in recombinant system. In this study, one of key regulators that affect the cell growth and PHB content was confirmed and analyzed.RESULT:
S17-3, a mutant E. coli strain derived from S17-1, was found to be able to achieve high cell density when expressing NphaCAB with the plasmid pBhya-CAB. Whole genome sequencing of S17-3 revealed genetic alternations on the upstream regions of csrA, encoding a global regulator cross-talking between stress response, catabolite repression and other metabolic activities. Deletion of csrA or expression of mutant csrA resulted in improved cell density and PHB content.CONCLUSION:
The impact of gene deletion of csrA was determined, dysfunction of the regulators improved the cell density of recombinant E. coli and PHB production, however, the detail mechanism needs to be further clarified.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Escherichia coli
/
Hidroxibutiratos
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Chile
/
China
Instituição/País de afiliação:
Chinese Academy of Science/CN
/
Universidad de Santiago de Chile/CL
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