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The role of pioglitazone in antioxidant, anti-inflammatory, and insulin sensitivity in a high fat-carbohydrate diet-induced rat model of insulin resistance
Al-Muzafar, H M; Alshehri, F S; Amin, K A.
  • Al-Muzafar, H M; College of Science, Imam Abdulrahman Bin Faisal University. Department of Chemistry. Dammam. SA
  • Alshehri, F S; College of Science, Imam Abdulrahman Bin Faisal University. Department of Chemistry. Dammam. SA
  • Amin, K A; College of Science, Imam Abdulrahman Bin Faisal University. Department of Chemistry. Dammam. SA
Braz. j. med. biol. res ; 54(8): e10782, 2021. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1249333
ABSTRACT
We explored the cascade effects of a high fat-carbohydrate diet (HFCD) and pioglitazone (an anti-diabetic therapy used to treat type 2 diabetes mellitus (T2DM)) on lipid profiles, oxidative stress/antioxidant, insulin, and inflammatory biomarkers in a rat model of insulin resistance. Sixty albino rats (80-90 g) were randomly divided into three dietary groups; 1) standard diet; 2) HFCD diet for 12 weeks to induce an in vivo model of insulin resistance; and 3) HFCD diet plus pioglitazone. Blood and tissue samples were taken to assess hepatic function, lipid profiles, oxidative biomarkers, malondialdehyde (MDA) levels, antioxidant defense biomarkers, including reduced glutathione (GSH), superoxide dismutase (SOD), and the inflammatory markers interleukin-6 (IL-6) and tumor necrotic factor (TNF-α). HFCD-fed rats had significantly (P≤0.05) increased serum triacylglycerol (TG), total cholesterol (TC), low-density lipoprotein (LDL), alanine transaminase (ALT), and bilirubin levels, but decreased high-density lipoprotein (HDL) levels compared with the normal group. Moreover, serum leptin, resistin, TNF-α, and IL-6 levels were increased significantly in HFCD animals compared with controls. Similarly, HFCD-induced insulin resistance caused antioxidant and cytokine disturbances, which are important therapy targets for pioglitazone. Importantly, administration of this drug ameliorated these changes, normalized leptin and resistin and inflammatory markers by reducing TNF-α levels. Metabolic cascades of elevated lipid profiles, oxidative stress, insulin, and inflammatory biomarkers are implicated in insulin resistance progression. HFCD induced metabolic cascades comprising hypertriglyceridemia, hyperglycemia, insulin resistance, obesity-associated hormones, and inflammatory biomarkers may be alleviated using pioglitazone.
Assuntos


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Resistência à Insulina / Diabetes Mellitus Tipo 2 Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Arábia Saudita Instituição/País de afiliação: College of Science, Imam Abdulrahman Bin Faisal University/SA

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Resistência à Insulina / Diabetes Mellitus Tipo 2 Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Arábia Saudita Instituição/País de afiliação: College of Science, Imam Abdulrahman Bin Faisal University/SA