A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
Braz. j. med. biol. res
;
54(9): e10700, 2021. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1249336
ABSTRACT
It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Queimaduras
/
Sapotaceae
Tipo de estudo:
Guia de Prática Clínica
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2021
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Departamento de Clínica Odontológica, Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal do Ceará/BR
/
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará/BR
/
Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará/BR
/
Embrapa Agroindustria Tropical/BR
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