Anaplasmosis in Uganda. II. Prevalence of bovine anaplasmosis in Uganda
Ann. trop. med. parasitol
;
85(3): 305-8, 1991.
Artigo
em Inglês
| AIM
| ID: biblio-1259299
ABSTRACT
The prevalence of bovine anaplasmosis was studied in 320 Zebu cattle randomly selected from three regions of Uganda (Central; Southwestern and Northwestern) using dot-ELISA; Western immunoblotting; rapid card agglutination test (RCAT); capillary tube agglutination test (CAT); complement fixation test (CFT); and parasitological techniques. Dried blood on Whatman filter paper No. 1 was eluted in PBS 0.05pc Tween 20 prior to testing at an initial dilution of 125. The prevalences of parasitaemia were 25pc in the central region; 28pc in the southwestern region; and 35pc in the northwestern region; and the serological prevalence was lowest in the central region and highest in the northwest. Overall; prevalence rates obtained by dot-ELISA (61.9pc) and Western immunoblotting (62.5pc) were 1.5 times those obtained by RCAT (41pc) and three times those obtained by CAT (22.5pc). The overall prevalence rates obtained by dot-ELISA and Western immunoblotting compared favourably with the CFT data. The present data utilizing dried blood on filter papers indicate that there is a high prevalence of anaplasmosis in those regions of Uganda surveyed; and confirm our observations and those of others that collecting blood on filter papers is a suitable technique for large scale screening and for seroepidemiological studies
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Índice:
AIM (África)
Assunto principal:
Testes de Aglutinação
/
Bovinos
/
Testes de Fixação de Complemento
/
Ensaio de Imunoadsorção Enzimática
/
Doenças dos Bovinos
/
Western Blotting
/
Prevalência
/
Anaplasmose
Tipo de estudo:
Estudo de prevalência
/
Fatores de risco
Idioma:
Inglês
Revista:
Ann. trop. med. parasitol
Ano de publicação:
1991
Tipo de documento:
Artigo
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