Validation of UV Spectrophotometric Method of Analysis of some Marketed Perfloxacin in Nigeria
West Sfr. J. Pharm
; 23(2): 51-56, 2012. tab
Article
em En
| AIM
| ID: biblio-1273588
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RESUMEN
Background:
Acquiring sophisticated LC instruments by most third world laboratories is capital intensive.Literatures on simple spectrophotometric analytical methods for pefloxacin are scarce.Objectives:
The present study was undertaken to develop and validate a simple and economic UV spectrophotometric method for estimating pefloxacin mesylate (PFM) in dosage preparations.Methods:
Using a JENWAY spectrophotometer at predetermined emax of 277nm with 1 v/v aqueous glacialacetic acid as blank; the method was validated for linearity; accuracy; precision; reproducibility; and specificity asper International Conference on Harmonization (ICH) guidelines and used to determine the content of pefloxacinin seven marketed brands in Nigeria.Results:
The method exhibited good linearity over a range of 0.40-12.0 ?g/ml (regression equation y = 0.0859x+0.0211 ; r=0.999). Mean recovery accuracy (99.183 ) and assay result in the range of 100.5- 110.17 for these lected brands were not significantly different at p=0.05. The coefficient of variation (CV) for both intra andinter-day were below 7 . The method was specific for pefloxacin in the presence of common excipientsConclusion:
The method gave good validation results and could be employed for routine analysis of PFM incommercial formulations
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Índice:
AIM
Assunto principal:
Espectrofotometria Atômica
/
Pefloxacina
Idioma:
En
Revista:
West Sfr. J. Pharm
Ano de publicação:
2012
Tipo de documento:
Article