C/EBPb converts bovine fibroblasts to adipocytes without hormone cocktail induction
Electron. j. biotechnol
;
52: 67-75, July. 2021. tab, graf, ilus
Artigo
em Inglês
| LILACS
| ID: biblio-1283594
ABSTRACT
BACKGROUND: Adipogenesis and fibrogenesis can be considered as a competitive process in muscle, which may affect the intramuscular fat deposition. The CCAAT/enhancer-binding protein beta (C/EBPb) plays an important role in adipogenesis, which is well-characterized in mice, but little known in bovine so far. RESULTS: In this study, real-time qPCR revealed that the level of C/EBPb was increased during the developmental stages of bovine and adipogenesis process of preadipocytes. Overexpression of C/EBPb promoted bovine fibroblast proliferation through mitotic clonal expansion (MCE), a necessary process for initiating adipogenesis, by significantly downregulating levels of p21 and p27 (p < 0.01). Also, the PPARc expression was inhibited during the MCE stage (p < 0.01). 31.28% of transfected fibroblasts adopted lipid-laden adipocyte morphology after 8 d. Real-time qPCR showed that C/EBPb activated the transcription of early stage adipogenesis markers C/EBPa and PPARc. Expression of ACCa, FASN, FABP4 and LPL was also significantly upregulated, while the expression of LEPR was weakened. CONCLUSIONS: It was concluded C/EBPb can convert bovine fibroblasts into adipocytes without hormone induction by initiating the MCE process and promoting adipogenic genes expression, which may provide new insights into the potential functions of C/EBPb in regulating intramuscular fat deposition in beef cattle.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Bovinos
/
Adipócitos
/
Proteína beta Intensificadora de Ligação a CCAAT
/
Fibroblastos
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2021
Tipo de documento:
Artigo
País de afiliação:
China
/
Paquistão
/
Arábia Saudita
Instituição/País de afiliação:
Northwest A&F University/CN
/
The University of Agriculture Peshawar/PK
/
University of Tabuk/SA
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