Preparation and in vitro release profiling of PLGA microspheres containing BSA as a model protein
Braz. J. Pharm. Sci. (Online)
;
56: e18748, 2020. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1285513
ABSTRACT
Conventional drug formulations are incapable of adequate delivery of proteins and peptides for therapeutic purposes. As these molecules have very short biological half-life, multiple dosing is required to achieve the desirable therapeutic effects. Microspheres are able to encapsulate proteins and peptide in the polymeric matrix while protecting them from enzymatic degradation. In this study Bovine Serum Albumin (BSA) matrix type microspheres were fabricated using Polylactide-co-glycolide (PLGA) by double emulsion solvent evaporation method. The effects of variables such as homogenizer speed, molecular weight of polymer and the effect of pH of the water phases, were investigated against factors such as drug loading, encapsulation efficiency, morphology, size, drug distribution and release profile of the microspheres. Results, suggested that an increase in homogenization speed leads to a decrease in microsphere size. The increase in homogenization speed also caused a significant effect on the release profile only when higher molecular weight of polymer had been used.. The pH change of the internal aqueous phase led to modification of surface morphology of spheres to a porous structure that significantly increased the total amount of released protein. Integrity of protein structure was intact as shown by SDS-PAGE. According to the results, it can be concluded that we achieved a reproducible method regarding controlled protein delivery for different sizes of particles.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Técnicas In Vitro
/
Preparações Farmacêuticas
/
Proteínas
/
Microesferas
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Irã
/
Nova Zelândia
Instituição/País de afiliação:
Alborz University of Medical Sciences/IR
/
Tehran University of Medical Sciences/IR
/
University of Auckland/NZ
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