Identification of Proteins Associated with the Formation of Oral Biofilms
Pesqui. bras. odontopediatria clín. integr
;
21: e0128, 2021. tab, graf
Artigo
em Inglês
| LILACS, BBO
| ID: biblio-1287490
ABSTRACT
ABSTRACT Objective:
To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material andMethods:
Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting.Results:
The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation.Conclusion:
HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fusobacterium nucleatum
/
Biofilmes
/
Genômica
/
Placa Dentária
/
Streptococcus gordonii
Tipo de estudo:
Estudo diagnóstico
/
Fatores de risco
País/Região como assunto:
América do Sul
/
Peru
Idioma:
Inglês
Revista:
Pesqui. bras. odontopediatria clín. integr
Assunto da revista:
Odontologia
Ano de publicação:
2021
Tipo de documento:
Artigo
País de afiliação:
Peru
Instituição/País de afiliação:
Universidad Nacional Mayor de San Marcos/PE
/
Universidad Peruana Cayetano Heredia/PE
/
Universidad Privada San Juan Bautista/PE
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