Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy
Acta cir. bras
;
36(11): e361104, 2021. tab, graf
Artigo
em Inglês
| LILACS, VETINDEX
| ID: biblio-1360061
ABSTRACT
ABSTRACT Purpose:
To investigate the underlying mechanism of hepatic sinusoidal obstruction syndrome (HSOS) induced by Gynura segetum by measuring autophagy in mouse models.Methods:
The model group was administered G. segetum (30 g/kg/d) by gavage, while the normal control group was administered an equal volume of saline daily for five weeks. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic histopathological examinations, and Masson staining were performed to evaluate liver injury. Liver intercellular adhesion molecule-1 (ICAM-1) and P-selectin were evaluated by immunohistochemistry. Hepatocellular apoptosis was assessed using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Protein expression levels of autophagy markers were measured using Western blot analysis.Results:
Gynura segetum was found to significantly induce liver injury compared with control mice, as evidenced by the increase of serum transaminases, a decrease in triglyceride levels, and histopathological changes in mice. Gynura segetum remarkably induced hepatocellular apoptosis and upregulated the expressions of ICAM-1 and P-selectin and also downregulated the protein expression levels of LC3, Atg12 and cytoplasmic polyadenylation element binding protein.Conclusions:
Our results suggested that G. segetum induced liver injury with HSOS, and it was partly due to its ability to impair the autophagy pathway.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Hepatopatia Veno-Oclusiva
/
Medicamentos de Ervas Chinesas
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Ano de publicação:
2021
Tipo de documento:
Artigo
Instituição/País de afiliação:
Faculty of Pharmacy/CN
/
Ningbo University/CN
/
Ningbo Yinzhou No.2 Hospital/CN
/
Wenzhou Medical University/CN
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