Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples
Braz. J. Pharm. Sci. (Online)
;
58: e19587, 2022. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1384015
ABSTRACT
This work aims to develop analytical methods using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins (AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and some parameters such as temperature, flow rate, injection volume and mobile phase with different proportions of solvents were evaluated. The best chromatographic conditions were obtained using the Phenomenex® C18 (150 × 4.60 mm, 5 µm) column at a temperature of 20 °C, flow rate of 1.2 mL min-1, injection volume of 20 µL, and detection at 250 nm. Acetonitrile ultrapure water (87 13, v/v) was used as mobile phase for moxidectin and eprinomectin, and acetonitrile methanol ultrapure water (53 35 12, v/v/v) for abamectin and ivermectin. Under these conditions satisfactory results were obtained, with appropriate limits of detection and quantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfy the need for analytical methods for the multi-determination of MBMs and the B1a and B1b forms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative and should aid in the development of the fiel
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Métodos de Análise Laboratorial e de Campo
/
Cromatografia Líquida de Alta Pressão
/
Estudo de Validação
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2022
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de São João del-Rei/BR
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