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Extended biofilm formation time by Streptococcus sanguinis modifies its non-cariogenic behavior, in vitro
DÍAZ-GARRIDO, Natalia; LOZANO, Carla Paola; KRETH, Jens; GIACAMAN, Rodrigo Andrés.
  • DÍAZ-GARRIDO, Natalia; University of Talca. Department of Oral Rehabilitation. Cariology Unit. Talca. CL
  • LOZANO, Carla Paola; Universidad de Chile. Faculty of Dentistry. Institute for Research in Dental Sciences. Santiago. CL
  • KRETH, Jens; Oregon Health & Science University. Department of Restorative Dentistry. Portland. US
  • GIACAMAN, Rodrigo Andrés; Chilean State Universities Consortium. Interuniversity Center on Healthy Aging. Santiago. CL
Braz. oral res. (Online) ; 36: e107, 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, BBO | ID: biblio-1394169
ABSTRACT
Abstract Although the commensal Streptococcus sanguinis [ S. sanguinis] is isolated from caries-free people, it can ferment carbohydrates producing acids. We aimed to characterize S. sanguinis cariogenic potential as a function of different enamel biofilm formation periods, in vitro. Saliva-coated enamel slabs were inoculated with S. sanguinis to form initial biofilms for 8, 12 or 16 h in presence of sucrose and followed by a period in medium with glucose for 16, 12 or 8 h, respectively, until completion of 24 h. To simulate cariogenic challenges, S. sanguinis biofilms were exposed to 10% sucrose for 5 minutes, 3x/day for 5 days. Biofilm biomass, viable cells, total proteins, intracellular and extracellular polysaccharides production, acidogenicity and enamel demineralization were determined. Biofilms of Streptococcus mutans [ S. mutans ] served as caries-positive control. Biofilms of S. sanguinis forming on enamel for 12 and 16 h showed higher demineralization than those formed during 8 h, but lower than S. mutans biofilms, regardless of the initial biofilm formation time. No differences were detected in the biofilm properties among the different biofilm formation times tested for S. sanguinis . Increased enamel initial biofilm formation time by S. sanguinis appears to induce a cariogenic potential, but lower than S. mutans .


Texto completo: DisponíveL Índice: LILACS (Américas) Idioma: Inglês Revista: Braz. oral res. (Online) Assunto da revista: Odontologia Ano de publicação: 2022 Tipo de documento: Artigo / Documento de projeto País de afiliação: Chile / Estados Unidos Instituição/País de afiliação: Chilean State Universities Consortium/CL / Oregon Health & Science University/US / Universidad de Chile/CL / University of Talca/CL

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Texto completo: DisponíveL Índice: LILACS (Américas) Idioma: Inglês Revista: Braz. oral res. (Online) Assunto da revista: Odontologia Ano de publicação: 2022 Tipo de documento: Artigo / Documento de projeto País de afiliação: Chile / Estados Unidos Instituição/País de afiliação: Chilean State Universities Consortium/CL / Oregon Health & Science University/US / Universidad de Chile/CL / University of Talca/CL