Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil
Rev. Soc. Bras. Med. Trop
; Rev. Soc. Bras. Med. Trop;55: e0067, 2022. tab, graf
Article
em En
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LILACS-Express
| LILACS
| ID: biblio-1406978
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Background:
Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear.Methods:
We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing.Results:
Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV).Conclusions:
The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence.
Texto completo:
1
Índice:
LILACS
País/Região como assunto:
America do sul
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Brasil
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2022
Tipo de documento:
Article
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Project document