Pin1 aggravates renal injury induced by ischemia and reperfusion in rats via Nrf2/HO-1 mediated endoplasmic reticulum stress
Acta cir. bras
;
37(1): e370101, 2022. ilus, graf
Artigo
em Inglês
| LILACS, VETINDEX
| ID: biblio-1413330
ABSTRACT
Purpose:
To investigate the role of peptidyl-prolyl cis/trans isomerase 1 (Pin1) on renal ischemia-reperfusion (I/R) injury and underlying mechanism.Methods:
By establishing the in vitro and in vivo models of renal I/R, the role of Pin1 was explored by using molecular assays.Results:
In renal I/R, endogenous Pin1 level was up-regulated in I/R-impaired kidney. Suppression of Pin1 with juglone afforded protection against I/R-mediated kidney dysfunction, and reduced I/R-induced endoplasmic reticulum (ER) stress in vivo. Consistent with the in vivo results, repression of Pin1 with juglone or gene knockdown with si-Pin1 conferred cytoprotection and restricted hypoxia/reoxygenation (H/R)-driven ER stress in HK-2 cells. Simultaneously, further study uncovered that Nrf-2/HO-1 signals was the association between Pin1 and ER stress in response to renal I/R. In addition, Nrf-2/HO-1 signal pathway was inactivated after kidney exposed to I/R, as indicated by the down-regulation of Nrf-2/HO-1 levels. Furthermore, inhibition of Pin1 remarkably rescued the inactivation ofNrf-2/HO-1.Conclusions:
Pin1 modulated I/R-mediated kidney injury in ER stress manner dependent on Nrf2-HO-1 pathway in I/R injury.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Heme Oxigenase-1
/
Fator 2 Relacionado a NF-E2
/
Peptidilprolil Isomerase de Interação com NIMA
/
Isquemia
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Huazhong University of Science and Technology/CN
/
The Fifth Hospital of Wuhan/CN
/
The First Affiliated Hospital of Anhui Medical University/CN
/
University of South China/CN
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