Effects of Chitosan Nanoparticles with Long Synthetic siRNAs Targeting VEGF in Triple-Negative Breast Cancer Cells
Braz. J. Pharm. Sci. (Online)
;
59: e22304, 2023. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1447564
ABSTRACT
Abstract Vascular endothelial growth factor (VEGF) is an essential angiogenic factor in breast cancer development and metastasis. Small interfering RNAs (siRNAs) can specifically silence genes via the RNA interference pathway, therefore were investigated as cancer therapeutics. In this study, we investigated the effects of siRNAs longer than 30 base pairs (bp) loaded into chitosan nanoparticles in triple-negative breast cancer cells, compared with conventional siRNAs. 35 bp long synthetic siRNAs inhibited VEGF gene expression by 51.2% and increased apoptosis level by 1.75-fold in MDA-MB-231 cell lines. Furthermore, blank and siRNA-loaded chitosan nanoparticles induced expression of IFN-γ in breast cancer cells. These results suggest that long synthetic siRNAs can be as effective as conventional siRNAs, when introduced into cells with chitosan nanoparticles
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
RNA Interferente Pequeno
/
Fator A de Crescimento do Endotélio Vascular
/
Quitosana
/
Nanopartículas
/
Neoplasias de Mama Triplo Negativas
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
Turquia
Instituição/País de afiliação:
Istanbul Medipol University/TR
/
Marmara University/TR
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