Neuroprotective effects of an Nrf2 agonist on high glucose-induced damage in HT22 cells
Biol. Res
;
52: 53-53, 2019. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: biblio-1505773
ABSTRACT
BACKGROUND: Oxidative stress is the hallmark of diabetic encephalopathy, which may be caused by hyperglycaemic toxicity. We aimed to discover pharmacologic targets to restore redox homeostasis. We identified the transcription factor Nrf2 as such a target. METHODS: HT22 cells were cultured in 25 or 50 mM D-glucose with various concentrations of sulforaphane (SFN) (from 1.25 to 5.0 µM). Cell viability was tested with the Cell Counting Kit-8 assay. Reactive oxygen species (ROS) production was detected with an inverted fluorescence microscope using the dichlorodihydrofluorescein-diacetate fluorescent probe. The expression of NF-E2-related factor 2 (Nrf2), haem oxygenase-1 (HO-1) and nuclear factor-κB (NF-κB) at the mRNA and protein levels was detected by reverse transcription quantitative polymerase chain reaction and western blotting. RESULT: We found that a high glucose concentration (50 mM) increased the generation of ROS, downregulated the expression of Nrf2/HO-1 and upregulated the expression of NF-κB. Moreover, HT22 cell viability significantly decreased after culture in high-glucose medium for 24, 48 and 72 h, whereas the activation of the Nrf2/HO-1 pathway using a pharmacological Nrf2 activator abrogated this high-glucose-induced toxicity. CONCLUSION: This study suggests that the activation of the Nrf2-ARE signalling pathway might be a therapeutic target for the treatment of diabetic encephalopathy.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fator 2 Relacionado a NF-E2
/
Neuroproteção
/
Glucose
/
Hipocampo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Henan Provincial People's Hospital/CN
/
The First Affiliated Hospital of Zheng-zhou University/CN
/
The Six Affiliated Hospital of Sun Yat-Sen University/CN
/
The Six Affiliated Hospital of Sun Yat-Sen University,/CN
/
Zhejiang Hospital/CN
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