Salivary Metabolites in Patients with Mucopolysaccharidosis
Pesqui. bras. odontopediatria clín. integr
;
24: e220151, 2024. graf
Artigo
em Inglês
| LILACS, BBO
| ID: biblio-1535004
ABSTRACT
ABSTRACT Objective:
To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material andMethods:
The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 730 and 900 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4°C, and the supernatants (500µl) were stored at −80°C until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively.Results:
Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring.Conclusion:
The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Saliva
/
Espectroscopia de Ressonância Magnética
/
Mucopolissacaridoses
/
Metabolômica
/
Espectroscopia de Prótons por Ressonância Magnética
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Pesqui. bras. odontopediatria clín. integr
Assunto da revista:
Odontologia
Ano de publicação:
2024
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of Rio de Janeiro/BR
/
State University of Rio de Janeiro/BR
Similares
MEDLINE
...
LILACS
LIS