Ultrasound as a safe and reliable guidance for subarachnoid puncture in rabbits
Acta cir. bras
;
32(1): 14-21, Jan. 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-837670
ABSTRACT
Abstract Purpose: To evaluate a model for studying the toxicity in nervous tissue and meninges using ultrasound to guide needle insertion into the subarachnoid space of rabbits, with the objective of avoiding injuries triggered by the puncture and by intraneural injection of solutions. Methods: Forty-five adult female rabbits were divided into 3 groups (G): G1 underwent subarachnoid puncture, G2 underwent subarachnoid injection of saline solution and G3 underwent subarachnoid injection of 0.5% hyperbaric bupivacaine. A needle was inserted into the S1-S2 subarachnoid space guided by ultrasound. The sensitivity and motility of the animals were evaluated for 3 days, after which the animals were sacrificed for removal of lumbar and sacral portions of the spinal cord for histological examination by light microscopy and immunohistochemistry. Results: All animals had motor function and pain sensitivity on the evaluation period. No animal had complication during or after the procedures. Conclusion: Ultrasound is a useful and safe method for the correct identification of the subarachnoid space of rabbits.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Punção Espinal
/
Espaço Subaracnóideo
/
Ultrassonografia de Intervenção
Tipo de estudo:
Guia de Prática Clínica
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual Paulista/BR
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