Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
Biol. Res
;
50: 4, 2017.
Artigo
em Inglês
| LILACS
| ID: biblio-838961
ABSTRACT
Abstract Background Macrophages are a functionally heterogeneous cell population and depending on microenvironments they polarize in two main groups M1 and M2. Glutamic acid and glutamate receptors may participate in the regulation of macrophage plasticity. To investigate the role of glutamatergic systems in macrophages physiology, we performed the transfection of mGluR5 cDNAs into RAW-264.7 cells. Results Comparative analysis of modified (RAW-mGluR5 macrophages) and non-modified macrophages (RAW-macrophages) has shown that the RAW-mGluR5 macrophages absorbed more glutamate than control cells and the amount of intracellular glutamate correlated with the expression of excitatory amino acid transporters -2 (EAAT-2). Besides, our results have shown that RAW-mGluR5 macrophages expressed a higher level of peroxisome proliferator-activated receptor γ (PPAR-γ) and secreted more IL-10, high mobility group box 1 proteins (HMGB1) and Galectin-3 than control RAW-macrophages. Conclusions We propose that elevation of intracellular glutamate and expression of mGluR5 may initiate the metabolic rearrangement in macrophages that could contribute to the formation of an immunosuppressive phenotype.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Receptor de Glutamato Metabotrópico 5
/
Plasticidade Celular
/
Macrófagos
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Geórgia
Instituição/País de afiliação:
Ilia State University/GE
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